Recombinant Human EXOSC1 293 Cell Lysate

• Cat.No.: EXOSC1-6505HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for exosome component 1 (EXOSC1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: EXOSC1 exosome component 1 [ Homo sapiens ]
• Official Symbol: EXOSC1
• Synonyms: EXOSC1; exosome component 1; exosome complex component CSL4; CGI 108; CSL4; CSL4 exosomal core protein homolog (yeast); Csl4p; hCsl4p; p13; SKI4; Ski4p; exosomal core protein CSL4; 3-5 exoribonuclease CSL4 homolog; CSL4 exosomal core protein homolog; homolog of yeast exosomal core protein CSL4; CGI-108; RP11-452K12.9
• Gene ID: 51013
• mRNA Refseq: NM_016046
• Protein Refseq: NP_057130
• MIM: 606493
• UniProt ID: Q9Y3B2
• Chromosome Location: 10q24
• Pathway: Activation of Genes by ATF4, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by KSRP, organism-specific biosystem; Destabilization of mRNA by Tristetraprolin (TTP), organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: RNA binding; NOT exoribonuclease activity; protein binding