Recombinant Human Eukaryotic Translation Elongation Factor 1 Delta (Guanine Nucleotide Exchange Protein), His-tagged

• Cat.No.: EEF1D-4998H
• Product Overview: Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: EEF1D-4998H
• Description: Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas.
• Concentration: 0.5 mg/ml
• Source: Human
• Host: E. coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.
• Purity: > 90% by SDS - PAGE
• Sequence: 1-281 amino acids: MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVA
• Molecular Mass: 33.2 kDa (301aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Applications: SDS - PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: EEF1D eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) [ Homo sapiens ]
• Official Symbol: EEF1D
• Synonyms: EEF1D; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); EF1D; EF-1D; FP1047; FLJ20897; elongation factor 1-delta; EF-1-delta; antigen NY-CO-4; OTTHUMP00000228834; OTTHUMP00000228835; OTTHUMP00000228854; OTTHUMP00000228855; OTTHUMP00000228856; OTTHUMP00000228857; OTTHUMP00000231014; OTTHUMP00000231272; OTTHUMP00000231355; OTTHUMP00000231356; OTTHUMP00000231357; OTTHUMP00000231358; OTTHUMP00000231359; OTTHUMP00000231460; guanine nucleotide exchange protein
• Gene ID: 1936
• mRNA Refseq: NM_032378
• Protein Refseq: NP_115754
• MIM: 130592
• UniProt ID: P29692
• Chromosome Location: 8q24
• Pathway: Eukaryotic Translation Elongation; Gene Expression; Metabolism of proteins; Translation; Translation Factors
• Function: protein binding; signal transducer activity; translation elongation factor activity; translation factor activity