Recombinant Human eukaryotic translation elongation factor 1 alpha 1, His-tagged

• Cat.No.: EEF1A1-516H
• Product Overview: EEF1A1, 1-462aa Human, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-462 a.a.
• Description: EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Recombinant human EEF1A1 protein is expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 50 kDa (462aa)
• AA Sequence: MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EEF1A1 eukaryotic translation elongation factor 1 alpha 1 [ Homo sapiens ]
• Official Symbol: EEF1A1
• Synonyms: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• Gene ID: 1915
• mRNA Refseq: NM_001402.5
• Protein Refseq: NP_001393.1
• MIM: 130590
• UniProt ID: P68104
• Chromosome Location: 6q14.1
• Pathway: EGFR1 Signaling Pathway, organism-specific biosystem;Eukaryotic Translation Elongation, organism-specific biosystem;Gene Expression, organism-specific biosystem;Legionellosis, organism-specific biosystem;Legionellosis, conserved biosystem;Metabolism of proteins, organism-specific biosystem;Peptide chain elongation, organism-specific biosystem
• Function: GTP binding;GTPase activity;protein binding;protein kinase binding;translation elongation factor activity