Recombinant Human ets homologous factor, His-tagged

• Cat.No.: EHF-544H
• Product Overview: EHF, 1-300aa, Human His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-300 a.a.
• Description: ETS homologous factor isoform 2, also known as EHF, is a distinct member of the ESE subfamily of Ets transcription factors. Ets factors constitute one important class of transcriptional regulators that play critical roles in hema-topoiesis, angiogenesis, organogenesis, oncogenesis and specification of neuronal connectivity. It is exclusively expressed in a subset of epithelial cells, with highest expression detected in glandular epithelium of the prostate, pancreas, salivary gland and trachea. EHF transactivates the c-Met promoter via three high affinity binding sites, which suggests that EHF may contribute to branching morphogenesis. Recombinant human EHF protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 37.3 kDa (323aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMILEGGG VMNLNPGNNL LHQPPAWTDS YSTCNVSSGF FGGQWHEIHP QYWTKYQVWE WLQHLLDTNQ LDANCIPFQE FDINGEHLCS MSLQEFTRAA GTAGQLLYSN LQHLKWNGQC SSDLFQSTHN VIVKTEQTEP SIMNTWKDEN YLYDTNYGST VDLLDSKTFC RAQISMTTTS HLPVAESPDM KKEQDPPAKC HTKKHNPRGT HLWEFIRDIL LNPDKNPGLI KWEDRSEGVF RFLKSEAVAQ LWGKKKNNSS MTYEKLSRAM RYYYKREILE RVDGRRLVYK FGKNARGWRE NEN
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EHF ets homologous factor [ Homo sapiens ]
• Official Symbol: EHF
• Synonyms: EHF; ets homologous factor; ETS homologous factor; epithelium specific ets factor 3; ESE3; ESE3 transcription factor; ESEJ; hEHF; ETS domain-containing transcription factor; epithelium-specific Ets transcription factor 3; ESE3B
• Gene ID: 26298
• mRNA Refseq: NM_001206615
• Protein Refseq: NP_001193544
• MIM: 605439
• UniProt ID: Q9NZC4
• Chromosome Location: 11p12
• Function: DNA binding; DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity