Recombinant Human ETF1 protein, His & T7-tagged

• Cat.No.: ETF1-1397H
• Product Overview: Recombinant Human ETF1 aa. (Ala2~Val221 (Accession # P62495) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.7kDa.
• Protein length: Ala2~Val221
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ETF1 eukaryotic translation termination factor 1 [ Homo sapiens (human) ]
• Official Symbol: ETF1
• Synonyms: ETF1; eukaryotic translation termination factor 1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1; eukaryotic peptide chain release factor subunit 1; polypeptide chain release factor 1; protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1
• Gene ID: 2107
• mRNA Refseq: NM_001256302.1
• Protein Refseq: NP_001243231.1
• UniProt ID: P62495