Recombinant Human ESR1, His-tagged

• Cat.No.: ESR1-27977TH
• Product Overview: Recombinant Human ESR1 was expressed in Baculovirus-insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.
• Form: Protein is stored in 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
• Molecular Mass: 67.9 kDa.
• AA Sequence: MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA VYNYPEGAAYEFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL NSVSPSPLML LHPPPQLSPFLQPHGQQVPY YLENEPSGYT VREAGPPAFY RPNSDNRRQG GRERLASTND KGSMAMESAKETRYCAVCND YASGYHYGVW SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQACRLRKCYEVGM MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKRSKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINWAKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG KLLFAPNLLL DRNQGKCVEGMVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLDKITDTLIHLM AKAGLTLQQQ HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLLLEMLDAHRLH APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV
• Purity: 0.9
• Applications: ER can be applied in DNA and protein-protein interaction assays. 1 unit is equal to 1 nanogram of purified protein. 20-100 units are sufficient for an in vitro transcription assay and 100 units are sufficient for a protein-protein interaction assay. For research use only.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: ESR1 estrogen receptor 1 [ Homo sapiens (human) ]
• Official Symbol: ESR1
• Synonyms: ESR1; estrogen receptor 1; ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123; Estrogen receptor; ER-alpha; Nuclear receptor subfamily 3 group A member 1
• Gene ID: 2099
• mRNA Refseq: NM_000125
• Protein Refseq: NP_000116
• MIM: 133430
• UniProt ID: P03372
• Chromosome Location: 6q25.1
• Pathway: Endocrine and other factor-regulated calcium reabsorption; Estrogen signaling pathway; FOXA1 transcription factor network
• Function: RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; beta-catenin binding