Recombinant Human ESAM protein(Met1-Ala248), hFc-tagged

• Cat.No.: ESAM-2328H
• Product Overview: Recombinant Human ESAM (NP_620411.2)(Met 1-Ala 248) was expressed in HEK293 with the fused Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-248 a.a.
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ESAM/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 457 amino acids and predicts a molecular mass of 50.5 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh ESAM/Fc monomer is approximately 65-70 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ESAM endothelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: ESAM
• Synonyms: ESAM; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; W117m; 2310008D05Rik; LP4791 protein; HUEL (C4orf1)-interacting protein
• Gene ID: 90952
• mRNA Refseq: NM_138961
• Protein Refseq: NP_620411
• MIM: 614281
• UniProt ID: Q96AP7