Recombinant Human ERI1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ERI1-3313H
• Product Overview: ERI1 MS Standard C13 and N15-labeled recombinant protein (NP_699163) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
• Molecular Mass: 40.1 kDa
• AA Sequence: MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASDFSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ERI1 exoribonuclease 1 [ Homo sapiens (human) ]
• Official Symbol: ERI1
• Synonyms: ERI1; exoribonuclease 1; THEX1, three prime histone mRNA exonuclease 1; 3-5 exoribonuclease 1; 3HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1 (C.elegans); Eri-1 homolog; protein 3hExo; 3 exoribonuclease; 3-5 exonuclease ERI1; histone mRNA 3-exonuclease 1; three prime mRNA exonuclease 1; three prime histone mRNA exonuclease 1; histone mRNA 3 end-specific exonuclease; histone mRNA 3-end-specific exoribonuclease; enhanced RNAi three prime mRNA exonuclease homolog 1; HEXO; THEX1; MGC35395
• Gene ID: 90459
• mRNA Refseq: NM_153332
• Protein Refseq: NP_699163
• MIM: 608739
• UniProt ID: Q8IV48