Recombinant Human ERF1 protein, GST-tagged

• Cat.No.: ERF1-317H
• Product Overview: Recombinant human ERF1 (2-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 2-end a.a.
• Description: Eukaryotic peptide chain release factor subunit 1 (eRF1) directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Molecular Mass: 74 kDa
• Purity: The purity of ERF1 was determined to be >95% by densitometry.
• Applications: Enzyme Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: eukaryotic translation termination factor 1 [ Homo sapiens (human) ]
• Official Symbol: ETF1
• Synonyms: ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
• Gene ID: 2107
• mRNA Refseq: NM_004730
• Protein Refseq: NP_004721
• MIM: 600282
• UniProt ID: P62495