Recombinant Human ERCC1, His-tagged

• Cat.No.: ERCC1-6778H
• Product Overview: Recombinant humanERCC1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ERCC1, also known asDNA excision repair protein ERCC-1, functions in the nucleotide excisionrepair pathway, and is required for the repair of DNA lesions such as thoseinduced by UV light or formed by electrophilic compounds including cisplatin.Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type4 (COFS4).
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Molecular Weight: 32.5 kDa (297aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 0.5 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAAPQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDVIPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTLILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLHEPFLKVP
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ERCC1excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence) [ Homo sapiens ]
• Official Symbol: ERCC1
• Synonyms: ERCC1; excision repaircross-complementing rodent repair deficiency, complementation group 1(includes overlapping antisense sequence); DNA excision repair proteinERCC-1; RAD10
• Gene ID: 2067
• mRNA Refseq: NM_001166049
• Protein Refseq: NP_001159521
• MIM: 126380
• Uniprot ID: P07992
• Chromosome Location: 19q13.32
• Pathway: DNA Repair, organism-specificbiosystem; Dual incision reaction in GG-NER, organism-specific biosystem;Dual incision reaction in TC-NER, organism-specific biosystem; Fanconi anemiapathway, organism-specific biosystem; Fanconi anemia pathway, conservedbiosystem
• Function: damaged DNA binding; damaged DNAbinding; endonuclease activity; hydrolase activity; protein C-terminusbinding