Recombinant Rat Erbb3 protein(Met1-His641), His-tagged

• Cat.No.: Erbb3-7437R
• Product Overview: Recombinant Rat ERBB3 (XP_017450189.1) (Met1-His641) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Protein length: 1-641 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human NRG1 Beta 1 Fc at 2 μg/ml (100 μl/well) can bind Rat HER3/ERBB3 His, the EC50 of Rat HER3/ERBB3 His is 100-400 ng/mL.
• Molecular Mass: The recombinant rat ERBB3 comprises 633 amino acids and predicts a molecular mass of 69.9 kDa. The apparent molecular mass of the recombinant protein is approximately 112 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Rattus norvegicus ]
• Official Symbol: Erbb3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); receptor tyrosine-protein kinase erbB-3; c-erbB3; c-erbB-3; avian erythroblastosis oncogene B 3; proto-oncogene-like protein c-ErbB-3
• Gene ID: 29496
• mRNA Refseq: NM_017218
• Protein Refseq: NP_058914