Recombinant Human ErbB3 protein, hFc-tagged

• Cat.No.: ErbB3-1398H
• Product Overview: Recombinant Human ErbB3 protein(P21860)(Ser20-Thr643), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Ser20-Thr643
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Her3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 95.4 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Homo sapiens ]
• Official Symbol: ErbB3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; MGC88033
• Gene ID: 2065
• mRNA Refseq: NM_001005915
• Protein Refseq: NP_001005915
• MIM: 190151
• UniProt ID: P21860