Active Recombinant Monkey ERBB3 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: ERBB3-1178RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rhesus (Macaca mulatta) ErbB3 (XP_001113953.2) extracellular domain (Met 1-Thr 643) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Monkey
• Source: HEK293
• Tag: Fc
• Protein Length: 865
• Form: Lyophilized
• Bio-activity: 1. Measured by its ability to human NRG1 (aa 20-241) in functional Elisa.
  2. Measured by its ability to human NRG1 (aa 177-241) in functional Elisa.
  3. Measured by its ability to human NRG1 (aa 20-241)-His in functional Elisa.
• Molecular Mass: The recombinant Rhesus ErbB3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 865 amino acids and predicts a molecular mass of 95.7 kDa. As a result of glycosylation, rhesus ErbB3/Fc monomer migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Ser 20
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 erb-b2 receptor tyrosine kinase 3 [ Macaca mulatta ]
• Official Symbol: ERBB3
• Synonyms: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3
• Gene ID: 711407