Recombinant Human ERBB2

• Cat.No.: ERBB2-2515H
• Product Overview: RecombinantHuman ERBB2 protein, was expressed in Human HEK293 cells and purified bySDS-PAGE and Coomassie blue staining

Specification

• Species: Human
• Tag: Non
• Description: This gene encodes amember of the epidermal growth factor receptor (EGFR) family of receptortyrosine kinases. This membrane-bound protein has a neuregulin binding domainbut not an active kinase domain. It therefore can bind this ligand but notconvey the signal into the cell through protein phosphorylation. However, itdoes form heterodimers with other EGF receptor family members which do havekinase activity. Heterodimerization leads to the activation of pathways whichlead to cell proliferation or differentiation. Amplification of this gene and/oroverexpression of its protein have been reported in numerous cancers,including prostate, bladder, and breast tumors. Alternate transcriptionalsplice variants encoding different isoforms have been characterized. Oneisoform lacks the intermembrane region and is secreted outside the cell. Thisform acts to modulate the activity of the membrane-bound form. Additionalsplice variants have also been reported, but they have not been thoroughlycharacterized.
• Preparation: HEK293Tcells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrsbefore collection. The cells were lysed in modified RIPA buffer (25mMTris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitorcocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify thelysate. Recombinant proteins were purified through anti-DDK affinity columns.Protein concentration was measured by BCA kit.
• Molecular Weight: 137.7 kDa
• Purity: > 80%as determined by SDS-PAGE and Coomassie blue staining
• Concentration: > 50ug/ml as determined by BCA
• Buffer: 10%glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
• Storage: Store at-80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt ofproducts under proper storage and handling conditions

Gene Information

• Gene Name: ERBB2v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblasticleukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogenehomolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; Receptortyrosine-protein kinase erbB-2; p185erbB2; C-erbB-2; Tyrosine kinase-typecell surface receptor HER2; MLN 19; c-erb B2/neu prote;neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblasticleukemia viral oncogene; homolog 2 (neuro/glioblastoma derived oncogenehomolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
• Gene ID: 2064
• mRNA Refseq: NM_004448
• Protein Refseq: NP_004439
• MIM: 164870
• UniProt ID: P04626
• Chromosome Location: 17q11.2-q12
• Pathway: Adherens junction; Bladder cancer;Calcium signaling pathway; Endometrial cancer; ErbB signaling pathway; Focaladhesion; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer;Prostate cancer; Axon guidance
• Function: ATP binding; ErbB-3 class receptorbinding; Hsp90 protein binding; epidermal growth factor receptor activity;glycoprotein binding; growth factor binding; identical protein binding;nucleotide binding; protein C-terminus binding; protein heterodimerizationactivity; protein phosphatase binding; protein phosphatase binding; receptorsignaling protein tyrosine kinase activity; transferase activity;transmembrane receptor activity; ubiquitin protein ligase bindin

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.