Recombinant Human Epstein-Barr virus induced 3 Protein and interleukin 27 Protein, Flag tagged

• Cat.No.: EBI3-250H
• Product Overview: Recombinant human IL-27 EBI-3 protein (Q14213) (Arg 21-Lys 229) linked human IL-27 P28 protein (Q8NEV9) (Phe 29-Pro 243) with a Flag tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• ProteinLength: EBI3: 21-229aa
  IL27: 29-243aa
• Description: Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. It is a heterodimeric cytokine that is encoded by two distinct genes, Epstein-Barr virus-induced gene 3 (EBI3) and IL-27p28. IL-27 is expressed by antigen presenting cells and interacts with a specific cell-surface receptor complex known as IL-27 receptor (IL-27R). This receptor consists of two proteins, IL-27Rɑ and gp130. IL-27 induces differentiation of the diverse populations of T cells in the immune system and also upregulates IL-10. When IL-27 binds to the IL-27 receptor, signaling pathways including JAK-STAT and p38 MAPK pathways are turned on.[6][2] There are two types of responses, pro-inflammatory and anti-inflammatory, which involve different types of cells, such as macrophages, dendritic cells, T cells, and B cells. The response that is activated is very much dependent on the external surrounding of IL-27. There are many different subsets of T cells, such as Th1, Th2, Th17, Tr1, and Treg cells; IL-27 is greatly involved in differentiation through inducing or suppressing of each T cell subset. Th1 cells, which express IFNγ, are generated by IL-27 through STAT1 dimerization and nuclear localization which subsequently leads to the expression of T-bet and signature Th1 genes. Th2 cells, which express IL-4, are inhibited by IL-27 through the transcription factor GATA-3. Th17 cells, which express IL-17, IL-22, and granulocyte macrophage colony-stimulating factor (GM-CSF), are inhibited by IL-27 through STAT1 and expression of transcription factor RORγt. Tr1 cells, which express IL-10, are induced by IL-27 through the transcription factor c-Maf. Treg cells are inhibited by IL-27 through STAT1 and STAT3.
• Tag: C-Flag
• Molecular Mass: 49.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.
• GeneID 2: 246778

Gene Information

• Gene Name: EBI3 Epstein-Barr virus induced 3 [ Homo sapiens (human) ]
• Official Symbol: EBI3
• Synonyms: EBI3; Epstein-Barr virus induced 3; IL27B; IL35B; IL-27B; interleukin-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; IL-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; epstein-Barr virus-induced gene 3 protein
• Gene ID: 10148
• mRNA Refseq: NM_005755
• Protein Refseq: NP_005746
• MIM: 605816
• UniProt ID: Q14213
• Gene Name 2: IL27 interleukin 27 [ Homo sapiens (human) ]
• Official Symbol 2: IL27
• Synonyms 2: IL27; interleukin 27; p28; IL30; IL-27; IL27A; IL-27A; IL27p28; interleukin-27 subunit alpha; IL-27 p28 subunit; IL-27 subunit alpha; IL-27-A; IL27-A; interleukin-30
• mRNA Refseq 2: NM_145659
• Protein Refseq 2: NP_663634
• MIM 2: 608273
• UniProt ID 2: Q8NEV9