Recombinant Human EPRS Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EPRS-2208H
• Product Overview: EPRS MS Standard C13 and N15-labeled recombinant protein (NP_004437) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 170.4 kDa
• AA Sequence: MATLSLTVNSGDPPLGALLAVEHVK DDVSISVEEGKENILHVSENVIFTD VNSILRYLARVATTAGLYGSNLMEH TEIDHWLEFSATKLSSCDSFTSTIN ELNHCLSLRTYLVGNSLSLADLCVW ATLKGNAAWQEQLKQKKAPVHVKRW FGFLEAQQAFQSVGTKWDVSTTKAR VAPEKKQDVGKFVELPGAEMGKVTV RFPPEASGYLHIGHAKAALLNQHYQ VNFKGKLIMRFDDTNPEKEKEDFEK VILEDVAMLHIKPDQFTYTSDHFET IMKYAEKLIQEGKAYVDDTPAEQMK AEREQRIDSKHRKNPIEKNLQMWEE MKKGSQFGQSCCLRAKIDMSSNNGC MRDPTLYRCKIQPHPRTGNKYNVYP TYDFACPIVDSIEGVTHALRTTEYH DRDEQFYWIIEALGIRKPYIWEYSR LNLNNTVLSKRKLTWFVNEGLVDGW DDPRFPTVRGVLRRGMTVEGLKQFI AAQGSSRSVVNMEWDKIWAFNKKVI DPVAPRYVALLKKEVIPVNVPEAQE EMKEVAKHPKNPEVGLKPVWYSPKV FIEGADAETFSEGEMVTFINWGNLN ITKIHKNADGKIISLDAKLNLENKD YKKTTKVTWLAETTHALPIPVICVT YEHLITKPVLGKDEDFKQYVNKNSK HEELMLGDPCLKDLKKGDIIQLQRR GFFICDQPYEPVSPYSCKEAPCVLI YIPDGHTKEMPTSGSKEKTKVEATK NETSAPFKERPTPSLNNNCTTSEDS LVLYNRVAVQGDVVRELKAKKAPKE DVDAAVKQLLSLKAEYKEKTGQEYK PGNPPAEIGQNISSNSSASILESKS LYDEVAAQGEVVRKLKAEKSPKAKI NEAVECLLSLKAQYKEKTGKEYIPG QPPLSQSSDSSPTRNSEPAGLETPE AKVLFDKVASQGEVVRKLKTEKAPK DQVDIAVQELLQLKAQYKSLIGVEY KPVSATGAEDKDKKKKEKENKSEKQ NKPQKQNDGQRKDPSKNQGGGLSSS GAGEGQGPKKQTRLGLEAKKEENLA DWYSQVITKSEMIEYHDISGCYILR PWAYAIWEAIKDFFDAEIKKLGVEN CYFPMFVSQSALEKEKTHVADFAPE VAWVTRSGKTELAEPIAIRPTSETV MYPAYAKWVQSHRDLPIKLNQWCNV VRWEFKHPQPFLRTREFLWQEGHSA FATMEEAAEEVLQILDLYAQVYEEL LAIPVVKGRKTEKEKFAGGDYTTTI EAFISASGRAIQGGTSHHLGQNFSK MFEIVFEDPKIPGEKQFAYQNSWGL TTRTIGVMTMVHGDNMGLVLPPRVA CVQVVIIPCGITNALSEEDKEALIA KCNDYRRRLLSVNIRVRADLRDNYS PGWKFNHWELKGVPIRLEVGPRDMK SCQFVAVRRDTGEKLTVAENEAETK LQAILEDIQVTLFTRASEDLKTHMV VANTMEDFQKILDSGKIVQIPFCGE IDCEDWIKKTTARDQDLEPGAPSMG AKSLCIPFKPLCELQPGAKCVCGKN PAKYYTLFGRSYTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EPRS glutamyl-prolyl-tRNA synthetase [ Homo sapiens (human) ]
• Official Symbol: EPRS
• Synonyms: EPRS; glutamyl-prolyl-tRNA synthetase; QARS, QPRS; bifunctional glutamate/proline--tRNA ligase; EARS; GLUPRORS; glutamate tRNA ligase; PARS; proline tRNA ligase; proline-tRNA ligase; prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; proliferation-inducing protein 32; bifunctional aminoacyl-tRNA synthetase; proliferation-inducing gene 32 protein; cell proliferation-inducing gene 32 protein; QARS; QPRS; PIG32; DKFZp313B047
• Gene ID: 2058
• mRNA Refseq: NM_004446
• Protein Refseq: NP_004437
• MIM: 138295
• UniProt ID: P07814