Recombinant Human Epidermal Growth Factor Receptor, GST-tagged

• Cat.No.: EGFR-1211H
• Product Overview: Recombinant humanEGFR (695-end) was expressed by baculovirus in Sf9 insect cells using anN-terminal GST tag.

Specification

• Cat. No.: EGFR-1211H
• Description: EGFR is the receptorfor members of the EGF family and is a transmembrane glycoprotein that hastyrosine kinase activity. Binding of epidermal growth factor to EGFR inducesreceptor dimerization and tyrosine autophosphorylation and leads to cellproliferation, differentiation, motility, and cell survival. Activation ofEGFR triggers mitogenic signaling in gastrointestinal mucosa, and itsexpression is upregulated in colon cancers and most neoplasms.
• Source: Sf9 insect cells
• Species: Human
• Purity: > 75% dens.
• Molecular Weight: 89 kDa
• Concentration: 0.1 mg/ml
• Endotoxin Level: Liquid in a bufferof 50 mM Tris-HCl, pH 7.5 + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mMEDTA + 0.1 mM PMSF + 25% Glycerol.
• Specific Activity: 60 nmol/min/mg
• Applications: Kinase Assay;Western Blot
• Storage: Store product at-80°C. For optimal storage, aliquot target into smaller quantities aftercentrifugation and store at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: EGFR
• Protein length: 695-end a.a.

Gene Information

• Gene Name: EGFR epidermal growth factorreceptor [ Homo sapiens ]
• Synonyms: EGFR; epidermalgrowth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; epidermalgrowth factor receptor; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663;OTTHUMP00000209210; OTTHUMP00000209211; proto-oncogene c-ErbB-1; cell growthinhibiting protein 40; cell proliferation-inducing protein 61; receptortyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b)oncogene homolog
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: Adherens junction;Alpha6-Beta4 Integrin Signaling Pathway; Androgen Receptor Signaling Pathway;Arf6 signaling events; Axon guidance; Bladder cancer; Calcium signalingpathway; Cytokine-cytokine receptor interaction; Developmental Biology;Dorso-ventral axis formation; E-cadherin signaling in keratinocytes; EGFRdownregulation; Endocytosis; Focal adhesion; Hepatitis C
• Function: ATP binding; MAP/ERKkinase kinase activity; actin filament binding; double-stranded DNA binding;enzyme binding; epidermal growth factor-activated receptor activity;identical protein binding; contributes_to nitric-oxide synthase regulatoractivity; nucleotide binding; protein binding; protein heterodimerizationactivity; protein phosphatase binding; protein tyrosine kinase activity;receptor activity; signal transducer activity