Recombinant Human EPHB4 protein, His-tagged, Biotinylated

• Cat.No.: EPHB4-356H
• Product Overview: Biotinylated Human EphB4 is expressed from human HEK293 cells. It contains AA Leu 16 - Ala 539 (Accession # NP_004435.3). It is the biotinylated form of Human EphB4 protein, His Tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 16 - Ala 539
• Description: Ephrin type-B receptor 4(EPHB4) is also known as HTK, MYK1 and TYRO11,is a member of Eph family. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EPHB4 binds to Ephrin-B2 and plays an essential role in vascular development. EPHB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EPHB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis and tumor growth. Incontrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EPHB4 is associated with prostate cancer and highly malignant breast cancers, accordingly, EPHB4 has potential application as a therapeutic candidate.
• Predicted N Terminal: Leu 16
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >97% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Storage buffer: PBS, pH7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: EPHB4 EPH receptor B4 [ Homo sapiens (human) ]
• Official Symbol: EPHB4
• Synonyms: HTK; MYK1; HFASD; TYRO11; ephrin receptor EphB4; hepatoma transmembrane kinase; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK
• Gene ID: 2050
• mRNA Refseq: NM_004444.4
• Protein Refseq: NP_004435.3
• UniProt ID: P54760