Recombinant Human EPHB4, His-tagged

• Cat.No.: EPHB4-28580TH
• Product Overview: Recombinant fragment, corresponding to amino acids 563-987 of Human Eph receptor B4 with an N-terminal His tag. MW: 50 kDa.

Specification

• Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
• Conjugation: HIS
• Tissue specificity: Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
• Biological activity: Specific Activity: 125 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: attggaaa ctgctgatct gaagtgggtg acattccctc aggtggacgg gcagtgggag gaactgagcg gcctggatga ggaacagcac agcgtgcgca cctacgaagt gtgtgacgtg cagcgtgccc cgggccaggc ccactggctt cgcacaggtt gggtcccacg gcggggcgcc gtccacgtgt acgccacgct gcgcttcacc atgctcgagt gcctgtccct gcctcgggct gggcgctcct gcaaggagac cttcaccgtc ttctactatg agagcgatgc ggacacggcc acggccctca cgccagcctg gatggagaac ccctacatca aggtggacac ggtggccgcg gagcatctca cccggaagcg ccctggggcc gaggccaccg ggaaggtgaa tgtcaagacg ctgcgtc
• Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.
• Protein length: 563-987 a.a.

Gene Information

• Gene Name: EPHB4 EPH receptor B4 [ Homo sapiens ]
• Official Symbol: EPHB4
• Synonyms: EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11
• Gene ID: 2050
• mRNA Refseq: NM_004444
• Protein Refseq: NP_004435
• MIM: 600011
• Uniprot ID: P54760
• Chromosome Location: 7q22
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; EPHB forward signaling, organism-specific biosystem; Ephrin B reverse signaling, organism-specific biosystem; EphrinB-EPHB pathway, organism-specific biosystem
• Function: ATP binding; ephrin receptor activity; ephrin receptor activity; nucleotide binding; protein binding