Recombinant Human EPHA4 protein(Ser570-Val986), His&GST-tagged

• Cat.No.: EPHA4-7315H
• Product Overview: Recombinant Human EPHA4 (P54764) (Ser570-Val986) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Protein length: 570-986 a.a.
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol, 3mM DTT
• Bio-activity: 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized human EPHA4 (aa 570-986) at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.
• Molecular Mass: The recombinant human EPHA4 /GST chimera consists of 654 amino acids and has a calculated molecular mass of 75 kDa. The recombinant protein migrates as an approximately 67 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA4 EPH receptor A4 [ Homo sapiens ]
• Official Symbol: EPHA4
• Synonyms: EPHA4; EPH receptor A4; EphA4 , TYRO1; ephrin type-A receptor 4; Hek8; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; SEK; HEK8; TYRO1
• Gene ID: 2043
• mRNA Refseq: NM_004438
• Protein Refseq: NP_004429
• MIM: 602188
• UniProt ID: P54764