Recombinant Human EPH Receptor A7, GST-His

• Cat.No.: EPHA7-745H
• Product Overview: Recombinant Human EPHA7, C-terminal fragment, amino acids G579-V998, activated, N-terminal GSTHIS6 fusion protein with a Thrombin and a 3C cleavage site, expressed inSf9insect cells. MW = 78,443 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 579-998 a.a.
• Description: EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
• Purification: GST-Affinity Chromatography.
• Activation: in vitro autoactivation.
• Product Identity: EPHA7 was confirmed as EPHA7 by mass spectroscopy LC-ESI-MS/MS.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.317 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 21 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: EPHA7 EPH receptor A7 [ Homo sapiens ]
• Synonyms: EPHA7; EPH receptor A7; EHK3; HEK11; ephrin type-A receptor 7; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3; EC 2.7.10.1
• Gene ID: 2045
• mRNA Refseq: NM_004440
• Protein Refseq: NP_004431
• MIM: 602190
• UniProt ID: Q15375
• Chromosome Location: 6q16.1
• Pathway: Axon guidance
• Function: ATP binding; chemorepellent activity; ephrin receptor activity; ephrin receptor binding; nucleotide binding; protein binding; protein tyrosine kinase activity; receptor activity; transferase activity; transmembrane receptor protein tyrosine kinase activity