Recombinant Human EPB49 Protein, His-tagged

• Cat.No.: EPB49-1429H
• Product Overview: Recombinant Human EPB49 Protein (Met1-Ser269) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Met1-Ser269
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: EPB49 erythrocyte membrane protein band 4.9 (dematin) [ Homo sapiens (human) ]
• Official Symbol: EPB49
• Synonyms: EPB49; erythrocyte membrane protein band 4.9 (dematin); dematin; DMT; FLJ78462; FLJ98848
• Gene ID: 2039
• mRNA Refseq: NM_001114135
• Protein Refseq: NP_001107607
• MIM: 125305
• UniProt ID: Q08495