Recombinant Human EP300 Protein, N-Flag-tagged

• Cat.No.: EP300-02H
• Product Overview: The wild-type p300 protein consists of full-length (2414 amino acid residues) p300 (accession number NM_001429) and contains an N-FLAG-Tag. The p300 protein was expressed in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.

Specification

• Description: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
• Source: Baculovirus
• Species: Human
• Tag: Flag
• Applications: Recombinant p300 is suitable for protein-protein interaction, in vitro transcription, in vitro acetylation and cell growth assays. 1-5 ng is sufficient for gelshift assays in a 20 μL volume, 50-100 ng is sufficient for reconstituted transcription assays and 100-200 ng is sufficient for protein-protein interaction or acetylation assays. The molecular weight of the protein is 265 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
• Notes: This product is guaranteed for 6 months from date of receipt.
• Stability: This product is guaranteed for 6 months from date of receipt.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.4 μg/μL
• Storage Buffer: 4 μg of Recombinant p300 protein in Dilution Buffer AM1 (20 mM TrisCl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA).

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens (human) ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; p300; KAT3B; MKHK2; RSTS2; histone acetyltransferase p300; E1A-associated protein p300; E1A-binding protein, 300kD; histone butyryltransferase p300; histone crotonyltransferase p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; EC 2.3.1.48
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q7Z6C1