Recombinant Human EP300 Protein (catalytic domain, 965-1810), N-Flag-tagged

• Cat.No.: EP300-04H
• Product Overview: Recombinant p300 protein, catalytic domain that includes amino acids 965 - 1810 of human p300 protein (accession number NP_001420.2) was expressed in baculovirus expression system and contains an N-FLAG tag.

Specification

• Description: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
• Source: Baculovirus
• Species: Human
• Tag: Flag
• Molecular Mass: 98.7 kDa
• Protein length: 965-1810
• Applications: Recombinant p300 protein, catalytic domain is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Usage: 0.5 μg Histone H4 was incubated with 0 μg (-), 0.5 μg (+) p300 protein, catalytic domain in 20 μL reaction system containing 50 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 50 ng/μL BSA, 1 mM TCEP and 20 μM Acetyl-CoA for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H4ac (pan-acetyl) antibody was used to recognize acetylated histone H4.
• Notes: This product is for research use only and is not for use in diagnostic procedures.
• Stability: This product is guaranteed for 6 months from date of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.23 μg/μL
• Storage Buffer: Recombinant p300 protein, catalytic domain is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens (human) ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; p300; KAT3B; MKHK2; RSTS2; histone acetyltransferase p300; E1A-associated protein p300; E1A-binding protein, 300kD; histone butyryltransferase p300; histone crotonyltransferase p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; EC 2.3.1.48
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q7Z6C1