Recombinant Human EMG1 Nucleolar Protein Homolog(S. cerevisiae), His-tagged

• Cat.No.: EMG1-6938H
• Product Overview: Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-244aa
• Description: EMG1 is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 29.1 kDa (267aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EMG1 EMG1 nucleolar protein homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: EMG1
• Synonyms: EMG1; EMG1 nucleolar protein homolog (S. cerevisiae); ribosomal RNA small subunit methyltransferase NEP1; C2F; Grcc2f; NEP1; essential for mitotic growth 1; ribosome biogenesis protein NEP1; 18S rRNA Psi1248 methyltransferase; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; FLJ60792
• Gene ID: 10436
• mRNA Refseq: NM_006331
• Protein Refseq: NP_006322
• MIM: 611531
• UniProt ID: Q92979
• Chromosome Location: 12p13
• Pathway: Ribosome biogenesis in eukaryotes, organism-specific biosystem; Ribosome biogenesis in eukaryotes, conserved biosystem
• Function: RNA binding; methyltransferase activity; rRNA (pseudouridine) methyltransferase activity; rRNA binding; transferase activity