Recombinant Human ELMO1 293 Cell Lysate

• Cat.No.: ELMO1-6622HCL

Specification

• Description: Antigen standard for engulfment and cell motility 1 (ELMO1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: ELMO1 engulfment and cell motility 1 [ Homo sapiens ]
• Official Symbol: ELMO1
• Synonyms: ELMO1; engulfment and cell motility 1; engulfment and cell motility 1 (ced 12 homolog, C. elegans); engulfment and cell motility protein 1; CED 12; CED12; ELMO 1; KIAA0281; ced-12 homolog 1; CED-12; ELMO-1; MGC126406
• Gene ID: 9844
• mRNA Refseq: NM_130442
• Protein Refseq: NP_569709
• MIM: 606420
• UniProt ID: Q92556
• Chromosome Location: 7p14.1
• Pathway: Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Disease, organism-specific biosystem; HIV Infection, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem
• Function: SH3 domain binding; protein binding