Recombinant Human EIF4EBP1 protein, His-tagged

• Cat.No.: EIF4EBP1-1845H
• Product Overview: Recombinant Human EIF4EBP1 aa. (Ser2-Ile118 (Accession # Q13541)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 13.97kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser2-Ile118
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >97%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4.
• Reconstitution: Reconstitute in PBS.

Gene Information

• Gene Name: EIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: EIF4EBP1
• Synonyms: EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; BP-1; 4EBP1; 4E-BP1; PHAS-I; eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• Gene ID: 1978
• mRNA Refseq: NM_004095.3
• Protein Refseq: NP_004086.1
• UniProt ID: Q13541