Recombinant Human EIF4A2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EIF4A2-4364H
• Product Overview: EIF4A2 MS Standard C13 and N15-labeled recombinant protein (NP_001958) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 46.4 kDa
• AA Sequence: MSGGSADYNREHGGPEGMDPDGVIESSWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EIF4A2 eukaryotic translation initiation factor 4A2 [ Homo sapiens (human) ]
• Official Symbol: EIF4A2
• Synonyms: EIF4A2; eukaryotic translation initiation factor 4A2; EIF4F, eukaryotic translation initiation factor 4A, isoform 2; eukaryotic initiation factor 4A-II; BM 010; DDX2B; EIF4A; ATP-dependent RNA helicase eIF4A-2; eukaryotic translation initiation factor 4A, isoform 2; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• Gene ID: 1974
• mRNA Refseq: NM_001967
• Protein Refseq: NP_001958
• MIM: 601102
• UniProt ID: Q14240