Recombinant Human EIF3J, GST-tagged

• Cat.No.: EIF3J-12371H
• Product Overview: Recombinant Human EIF3J protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-258a.a.
• Description: Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: EIF3J eukaryotic translation initiation factor 3, subunit J [ Homo sapiens ]
• Official Symbol: EIF3J
• Synonyms: EIF3J; eukaryotic translation initiation factor 3, subunit J; EIF3S1, eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 3 subunit J; eIF3 alpha; eIF3 p35; eIF3j; eIF-3-alpha; eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); EIF3S1; eIF3-p35; eIF3-alpha
• Gene ID: 8669
• mRNA Refseq: NM_003758
• Protein Refseq: NP_003749
• MIM: 603910
• UniProt ID: O75822
• Chromosome Location: 15q21.1
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: protein binding; contributes_to translation initiation factor activity