Recombinant Human EIF2B1, His-tagged

• Cat.No.: EIF2B1-3511H
• Product Overview: Recombinant human EIF2B1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-305aa).

Specification

• Cat. No.: EIF2B1-3511H
• Description: EIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Phosphorylation of eIF2 inhibits GEF activity of EIF2B, an inhibition that requires the eIF2B1 subunit. Defects in eIF2B1 are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease that is characterized by head trauma and motor deterioration.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Weight: 35.8 kDa(325aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLD AAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFP LNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDEL IKLYL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: EIF2B1 eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa [ Homo sapiens ]
• Synonyms: eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kD; EIF2B; EIF2BA; MGC117409; MGC125868; MGC125869; EIF2B1; translation initiation factor eIF-2B subunit alpha; eIF-2B GDP-GTP exchange factor subunit alpha
• Gene ID: 1967
• mRNA Refseq: NM_001414
• Protein Refseq: NP_001405
• UniProt ID: Q14232
• Chromosome Location: 12q24.31
• Function: GTP binding; enzyme regulator activity; protein binding; contributes_toguanyl-nucleotide exchange factor activity; contributes_to translation initiation factor activity