Recombinant Human EIF2AK3

• Cat.No.: EIF2AK3-30644TH
• Product Overview: Recombinant cytoplasmic domain of Human PERK (amino acids 563-1115) with N terminal proprietary tag; 552 amino acids, MWt 115kDa; Genbank Number:

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 554 amino acids
• Description: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
• Molecular Weight: 115.000kDa inclusive of tags
• Tissue specificity: Ubiquitous. A high level expression is seen in secretory tissues.
• Biological activity: The specific activity of EIF2AK3-30644TH was determined to be 18 nmol/min/mg by activity assay.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: NKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFATKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN
• Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3 [ Homo sapiens ]
• Official Symbol: EIF2AK3
• Synonyms: EIF2AK3; eukaryotic translation initiation factor 2-alpha kinase 3; PEK; PERK
• Gene ID: 9451
• mRNA Refseq: NM_004836
• Protein Refseq: NP_004827
• MIM: 604032
• Uniprot ID: Q9NZJ5
• Chromosome Location: 2p12
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Diabetes pathways, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem
• Function: ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; eukaryotic translation initiation factor 2alpha kinase activity; identical protein binding; nucleotide binding