Active Recombinant Human EHMT1 protein, GST-tagged

• Cat.No.: EHMT1-181H
• Product Overview: Recombinant Human EHMT1(895-end) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 895-end a.a.
• Description: Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is in charge of the mono- and demethylation of lysine-9 of histone H3. EHMT1 is found in a multimeric protein complex containing chromatin modifiers, which contributes to silencing of MYC- and E2F-responsive genes during G0 phase of cell cycle. EHMT1 plays a role in epithelial-to-mesenchymal transition and tumor invasion by repressing expression of tumour suppressor genes. Additionally, EHMT1 also methylates non-histone proteins such as lysine-373 of p53.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 380 nmol/min/mg
• Molecular Mass: 74 kDa
• Purity: >95%
• Applications: Methyltransferase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl
• Publications:
  The SETD6 Methyltransferase Plays an Essential Role in Hippocampus-Dependent Memory Formation (2019)

Gene Information

• Gene Name: EHMT1 euchromatic histone-lysine N-methyltransferase 1 [ Homo sapiens ]
• Official Symbol: EHMT1
• Synonyms: EHMT1; euchromatic histone-lysine N-methyltransferase 1; euchromatic histone methyltransferase 1; histone-lysine N-methyltransferase EHMT1; bA188C12.1; Eu HMTase1; FLJ12879; KIAA1876; KMT1D; H3-K9-HMTase 5; G9a like protein; G9a-like protein 1; lysine N-m
• Gene ID: 79813
• mRNA Refseq: NM_024757
• Protein Refseq: NP_079033
• MIM: 607001
• UniProt ID: Q9H9B1
• Chromosome Location: 9
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding