Recombinant Human EGLN1, MYC/DDK-tagged

• Cat.No.: EGLN1-127H
• Product Overview: Recombinant Human EGLN1, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
• Source: HEK293 cells
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 45.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: EGLN1 egl-9 family hypoxia-inducible factor 1 [ Homo sapiens (human) ]
• Official Symbol: EGLN1
• Synonyms: EGLN1; egl nine homolog 1 (C. elegans); C1orf12, EGL nine (C.elegans) homolog 1; egl nine homolog 1; HIF prolyl hydroxylase 2; HIFPH2; PHD2; SM 20; ZMYND6; egl nine-like protein 1; HIF-prolyl hydroxylase 2; zinc finger MYND domain-containing protein 6; hypoxia-inducible factor prolyl hydroxylase 2; prolyl hydroxylase domain-containing protein 2; HPH2; SM20; ECYT3; HPH-2; C1orf12; HIF-PH2; DKFZp761F179
• Gene ID: 54583
• mRNA Refseq: NM_022051
• Protein Refseq: NP_071334
• MIM: 606425
• UniProt ID: Q9GZT9
• Chromosome Location: 1q42.1
• Pathway: Cellular response to hypoxia; HIF-1 signaling pathway; HIF-2-alpha transcription factor network
• Function: L-ascorbic acid binding; peptidyl-proline 4-dioxygenase activity; peptidyl-proline dioxygenase activity