Recombinant Human EGFR Protein, His-tagged

• Cat.No.: EGFR-025H
• Product Overview: Recombinant human EGFR(rhEGFR) Met 1 - Lys 642 (Accession # NP_005219 ) fused with C terminal 6×His tag was expressed in human 293 cells (HEK293).

Specification

• Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
• Source: HEK293 cells
• Species: Human
• Tag: His
• Molecular Mass: rhEGFR contains C terminal 6×His Tag and has a calculated MW of 69.5 kDa. The predicted N-terminal is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.
• Endotoxin: Less than 0.01 ng endotoxin/1 µg rhEGFR.
• Purity: >95% purity as determined by SDS-PAGE of reduced rhEGFR.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhEGFR should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
• Protein length: 1-642 a.a.
• Publications:
  Discovery of Cyclic Peptidomimetic Ligands Targeting the Extracellular Domain of EGFR (2021)

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533