Recombinant Human EGFR Protein, Fc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: EGFR-024HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human EGFR Fc Chimera(rhEGFR-Fc) Met1-Lys642 (Accession # NP_005219) was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 860
• Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
• Molecular Mass: rhEGFR-Fc is a homodimeric protein and is fused with the Fc region of human IgG1 at the C-terminus. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. In DTT-reduced SDS-PAGE, protein migrates as 130-140 kDa.
• N-terminal Sequence Analysis: Leu 25
• Endotoxin: < 0.01 ng endotoxin/1 μg rhEGFR-Fc.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhEGFR-Fc should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533