Recombinant Human EFNB2 protein, hFc-tagged

• Cat.No.: EFNB2-8464H
• Product Overview: Recombinant Human EFNB2 protein(NP_004084.1)(Ile28-Ala229), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Ile28-Ala229
• Tag: C-hFc
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human EPHB2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB2 ephrin-B2 [ Homo sapiens ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L
• Gene ID: 1948
• mRNA Refseq: NM_004093
• Protein Refseq: NP_004084
• MIM: 600527
• UniProt ID: P52799