Recombinant Human EFNB2 protein, hFc-tagged

• Cat.No.: EFNB2-8464H
• Product Overview: Recombinant Human EFNB2 protein(NP_004084.1)(Ile28-Ala229), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Ile28-Ala229
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human EPHB2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB2 ephrin-B2 [ Homo sapiens ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L
• Gene ID: 1948
• mRNA Refseq: NM_004093
• Protein Refseq: NP_004084
• MIM: 600527
• UniProt ID: P52799

Reviews

01/05/2022

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

01/02/2022

The EFNB2 proteins purchased from your company is suitable for WB.

01/23/2021

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What effect does EphB6 overexpression have on EFNB2?

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in?

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2?

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed?

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells?

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2?

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.