Recombinant Human EED protein, His-tagged

• Cat.No.: EED-316H
• Product Overview: Recombinant full-length human EED was expressed in E.coli cells using an N-terminal His tag.

Specification

• Description: The embryonic ectoderm development protein (EED), a WD40 domain-containing protein, is the regulatory subunit of polycomb repressive complex 2 (PRC2). PRC2 catalyzes the monomethylation, dimethylation, and trimethylation of histone H3 Lys27 (H3K27) and acts as a central epigenetic regulator that marks the repressive chromatin domain. EED binds to the tri-methylated lysine 27 of the histone H3 (H3K27me3), and through which stimulates the activity of PRC2 allosterically.
• Source: E.coli
• Species: Human
• Tag: His
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
• Molecular Mass: 57 kDa
• Purity: The purity of EED was determined to be >90% by densitometry.
• Applications: Enzyme Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: embryonic ectoderm development [ Homo sapiens (human) ]
• Official Symbol: EED
• Synonyms: HEED; WAIT1
• Gene ID: 8726
• mRNA Refseq: NM_003797
• Protein Refseq: NP_003788
• MIM: 605984
• UniProt ID: O75530