Recombinant Human Ectodysplasin A, FLAG-tagged

• Cat.No.: EDA-2082H
• Product Overview: Recombinant Human EDA is produced inHEK 293 Cells. The extracellular domain of human EDA-A2 (ectodysplasin-A2) (aa 245-391) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG-tag. MW=28kDa (SDS-PAGE).

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 245-391 a.a.
• Description: EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Multiple alternatively spliced transcript variants have been found for this gene, but some variants lack sufficient support.
• Purity: ≥90% (SDS-PAGE).
• Concentration: 0.1mg/ml after reconstitution.
• Formulation: Lyophilized. Contains PBS.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
• Specificity: Binds to human XEDAR (X-linked ectodysplasin-A2 receptor).
• Stability: Stable for at least 6 months after receipt when stored at -20°C.

Gene Information

• Gene Name: EDA ectodysplasin A [ Homo sapiens ]
• Synonyms: EDA; ectodysplasin A; ED1; HED; EDA1; EDA2; XHED; XLHED; ED1-A1; ED1-A2; STHAGX1; ectodysplasin-A; ectodermal dysplasia protein; X-linked anhidroitic ectodermal dysplasia protein
• Gene ID: 1896
• mRNA Refseq: NM_001005609
• Protein Refseq: NP_001005609
• MIM: 300451
• UniProt ID: Q92838
• Chromosome Location: Xq12-q13.1
• Pathway: Cytokine-cytokine receptor interaction
• Function: protein binding; receptor binding; tumor necrosis factor receptor binding