Recombinant Human ECSIT Homolog (Drosophila), His-tagged

• Cat.No.: ECSIT-7177H
• Product Overview: Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 24.6 kDa.

Specification

• Description: ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT.
• Molecular Mass: 24.6 kDa (222aa) confirmed by MALDI-TOF
• Protein length: 19-217aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ECSIT ECSIT homolog (Drosophila) [ Homo sapiens ]
• Official Symbol: ECSIT
• Synonyms: ECSIT; ECSIT homolog (Drosophila); evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; signaling intermediate in Toll pathway evolutionarily conserved ortholog (mouse); SITPEC; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved
• Gene ID: 51295
• mRNA Refseq: NM_001142464
• Protein Refseq: NP_001135936
• MIM: 608388
• UniProt ID: Q9BQ95
• Chromosome Location: 19p13.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem
• Function: oxidoreductase activity, acting on NADH or NADPH; protein binding