Recombinant Human Early Growth Response 1

• Cat.No.: EGR1-2120H
• Product Overview: Recombinant Human Early Growth Response 1 was produced inSf9 cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
• Formulation: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
• Quantity: 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions.
• Specific Activity: >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
• Specificity: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
• Application: EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
• Storage: Store at -80℃. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: EGR1 early growth response 1 [ Homo sapiens ]
• Synonyms: EGR1; early growth response 1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; early growth response protein 1; nerve growth factor-induced protein A; KROX24;Protein Krox-243
• Gene ID: 1958
• mRNA Refseq: NM_001964
• Protein Refseq: NP_001955
• MIM: 128990
• UniProt ID: P18146
• Chromosome Location: 5q31.1
• Pathway: Prion diseases
• Function: DNA binding; double-stranded DNA binding; metal ion binding; protein binding; sequence-specific DNA binding; transcription activator activity; transcription factor activity; zinc ion binding