Recombinant Human E2F1, MYC/DDK-tagged

• Cat.No.: E2F1-238H
• Product Overview: Recombinant Human E2F1 fussed with MYC/DDK tag at C-terminal was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: E2F1 E2F transcription factor 1 [ Homo sapiens ]
• Official Symbol: E2F1
• Synonyms: RBP3; E2F-1; RBAP1; RBBP3; transcription factor E2F1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3
• Gene ID: 1869
• mRNA Refseq: NM_005225
• Protein Refseq: NP_005216
• MIM: 189971
• UniProt ID: Q01094
• Chromosome Location: 20q11.2
• Pathway: Activation of BH3-only proteins, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Apoptosis, organism-specific biosystem
• Function: DNA binding; core promoter binding; sequence-specific DNA binding