Recombinant Mouse E2f1 Protein, Myc/DDK-tagged

• Cat.No.: E2f1-2707M
• Product Overview: Purified recombinant protein of mouse full-length E2F transcription factor 1 (E2f1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Positively regulates transcription of RRP1B.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 46.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: E2f1 E2F transcription factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: E2f1
• Synonyms: E2F1; E2F transcription factor 1; transcription factor E2F1; E2F-1; mKIAA4009; KIAA4009
• Gene ID: 13555
• mRNA Refseq: NM_007891
• Protein Refseq: NP_031917
• UniProt ID: Q61501