Recombinant Human DSG3 Protein, C-His-tagged

• Cat.No.: DSG3-12H
• Product Overview: Recombinant Human DSG3 Protein, fused to His-tag at C-terminus, was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Glu24-Lys499
• Description: Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain. Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells. It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues. During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment. The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential. Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion. Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by the ability of immobilized Recombinant Human Desmoglein‑3 His-tag to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.750-6.00 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 56 kDa & 53 kDa
  SDS-PAGE: 57-67 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.

Gene Information

• Gene Name: DSG3 desmoglein 3 [ Homo sapiens (human) ]
• Official Symbol: DSG3
• Synonyms: DSG3; Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; pemphigus vulgaris antigen; PVA; ABOLM
• Gene ID: 1830
• mRNA Refseq: NM_001944.3
• Protein Refseq: NP_001935.2
• MIM: 169615
• UniProt ID: P32926