Recombinant Human DRD2 293 Cell Lysate

• Cat.No.: DRD2-6817HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for dopamine receptor D2 (DRD2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DRD2 dopamine receptor D2 [ Homo sapiens ]
• Official Symbol: DRD2
• Synonyms: DRD2; dopamine receptor D2; D(2) dopamine receptor; dopamine D2 receptor; dopamine receptor D2 isoform; seven transmembrane helix receptor; D2R; D2DR
• Gene ID: 1813
• mRNA Refseq: NM_000795
• Protein Refseq: NP_000786
• MIM: 126450
• UniProt ID: P14416
• Chromosome Location: 11q22-q23
• Pathway: Amine ligand-binding receptors, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cocaine addiction, organism-specific biosystem; Cocaine addiction, conserved biosystem; Dopamine receptors, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem
• Function: G-protein coupled receptor activity; dopamine binding; dopamine receptor activity, coupled via Gi/Go; dopamine receptor activity, coupled via Gi/Go; drug binding; drug binding; ionotropic glutamate receptor binding; potassium channel regulator activity; protein binding; receptor activity; receptor binding; signal transducer activity