Recombinant Human DPYSL3 293 Cell Lysate

Description :	Antigen standard for dihydropyrimidinase-like 3 (DPYSL3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	DPYSL3 dihydropyrimidinase-like 3 [ Homo sapiens ]
Official Symbol	DPYSL3
Synonyms	DPYSL3; dihydropyrimidinase-like 3; dihydropyrimidinase-related protein 3; CRMP4; DRP 3; ULIP; unc-33-like phosphoprotein 1; collapsin response mediator protein 4 long; DRP3; DRP-3; LCRMP; CRMP-4; ULIP-1;
Gene ID	1809
mRNA Refseq	NM_001197294
Protein Refseq	NP_001184223
MIM	601168
UniProt ID	Q14195
Chromosome Location	5q32
Pathway	Axon guidance, organism-specific biosystem; CRMPs in Sema3A signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
Function	SH3 domain binding; chondroitin sulfate binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides;