Recombinant Human DPH2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DPH2-2839H
• Product Overview: DPH2 MS Standard C13 and N15-labeled recombinant protein (NP_001375) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 52.1 kDa
• AA Sequence: MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DPH2 diphthamide biosynthesis 2 [ Homo sapiens (human) ]
• Official Symbol: DPH2
• Synonyms: DPH2; DPH2 homolog (S. cerevisiae); diptheria toxin resistance protein required for diphthamide biosynthesis like 2 (S. cerevisiae), DPH2 like 2 (S. cerevisiae), DPH2L2; diphthamide biosynthesis protein 2; DPH2-like 2; diphthamide biosynthesis-like protein 2; diphthamide biosynthesis protein 2 homolog-like 2; diptheria toxin resistance protein required for diphthamide biosynthesis-like 2; DPH2L2
• Gene ID: 1802
• mRNA Refseq: NM_001384
• Protein Refseq: NP_001375
• MIM: 603456
• UniProt ID: Q9BQC3