Recombinant Human Doublecortin-like Kinase 1, GST-tagged

• Cat.No.: DCLK1-1009H
• Product Overview: Full-length recombinant human DCLK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.

Specification

• Cat. No.: DCLK1-1009H
• Description: DCLK1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions). DCLK1 is a microtubule-associated kinase that can undergo autophosphorylation. DCLK1 has microtubule-polymerizing activity that is independent of its protein kinase activity. DCLK1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 110 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 3.4 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: DCLK1 doublecortin-like kinase 1 [ Homo sapiens ]
• Official Symbol: DCLK1
• Synonyms: DCLK1; doublecortin-like kinase 1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1; KIAA0369; serine/threonine-protein kinase DCLK1; OTTHUMP00000018250; OTTHUMP00000018251; OTTHUMP00000018252; doublecortin and CaM kinase-like 1; doublecortin-like and CAM kinase-like 1; doublecortin domain-containing protein 3A; EC=2.7.11.1
• Gene ID: 9201
• mRNA Refseq: NM_004734
• Protein Refseq: NP_004725
• MIM: 604742
• UniProt ID: O15075
• Chromosome Location: 13q13.3
• Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; receptor signaling protein activity