Recombinant Human DOK4, His-tagged

• Cat.No.: DOK4-5099H
• Product Overview: Recombinant human DOK4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. This protein is putative link with downstream effectors of RET in neuronal differentiation. DOK4 may be involved in the regulation of the immune response induced by T-cells.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
• Molecular Mass: 39.4kDa (349aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATNFSD IVKQGYVKMK SRKLGIYRRC WLVFRKSSSK GPQRLEKYPD EKSVCLRGCP KVTEISNVKC VTRLPKETKR QAVAIIFTDD SARTFTCDSE LEAEEWYKTL SVECLGSRLN DISLGEPDLL APGVQCEQTD RFNVFLLPCP NLDVYGECKL QITHENIYLW DIHNPRVKLV SWPLCSLRRY GRDATRFTFE AGRMCDAGEG LYTFQTQEGE QIYQRVHSAT LAIAEQHKRV LLEMEKNVRL LNKGTEHYSY PCTPTTMLPR SAYWHHITGS QNIAEASSYA GEGYGAAQAS SETDLLNRFI LLKPKPSQGD SSEAKTPSQ
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DOK4 docking protein 4 [ Homo sapiens ]
• Official Symbol: DOK4
• Synonyms: DOK4; docking protein 4; FLJ10488; IRS5; IRS-5; insulin receptor substrate 5; downstream of tyrosine kinase 4
• Gene ID: 55715
• mRNA Refseq: NM_018110
• Protein Refseq: NP_060580
• MIM: 608333
• UniProt ID: Q8TEW6
• Chromosome Location: 16q13
• Pathway: Signaling events regulated by Ret tyrosine kinase, organism-specific biosystem
• Function: insulin receptor binding; receptor signaling protein activity