Recombinant Human DNMT3L, GST-tagged

• Cat.No.: DNMT3L-6951H
• Product Overview: Recombinant full-length human DNMT3L was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: DNMT3L is an enzymatically inactive regulatory factor that shares sequence homology with the DNA methyltransferases DNMT3A and DNMT3B. DNMT3L is involved in the establishment of DNA methylation patterns during gametogenesis. In mammals, DNA methylation is used for heritable silencing of retrotransposons and imprinted genes and for inactivation of the X chromosome in females. DNMT3L has a genomewide role in CpG island methylation beyond genomic imprinting. DNMT3L functions as a regulator of methylation at imprinted loci rather than a DNA cytosine methyltransferase because of a lack of catalytic motifs in its sequence.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~70 kDa
• Protein length: Full length
• Purity: >80%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μ

Gene Information

• Gene Name: DNMT3L DNA (cytosine-5-)-methyltransferase 3-like [ Homo sapiens ]
• Official Symbol: DNMT3L
• Synonyms: DNMT3L; DNA (cytosine-5-)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; cytosine 5 methyltransferase 3 like protein; human cytosine 5 methyltransferase 3 like protein; MGC1090; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
• Gene ID: 29947
• mRNA Refseq: NM_013369
• Protein Refseq: NP_037501
• MIM: 606588
• UniProt ID: Q9UJW3
• Chromosome Location: 21q22.3
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem
• Function: enzyme activator activity; enzyme binding; metal ion binding; protein binding