Recombinant Full Length Human DNMT3L Protein, C-Flag-tagged

• Cat.No.: DNMT3L-1897HFL
• Product Overview: Recombinant Full Length Human DNMT3L Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 43.4 kDa
• AA Sequence: MAAIPALDPEAEPSMDVILVGSSEL SSSVSPGTGRDLIAYEVKANQRNIE DICICCGSLQVHTQHPLFEG GICAPCKDKFLDALFLYDDDGYQSY CSICCSGETLLICGNPDCTRCYCFE CVDSLVGPGTSGKVHAMSNW VCYLCLPSSRSGLLQRRRKWRSQLK AFYDRESENPLEMFETVPVWRRQPV RVLSLFEDIKKELTSLGFLE SGSDPGQLKHVVDVTDTVRKDVEEW GPFDLVYGATPPLGHTCDRPPSWYL FQFHRLLQYARPKPGSPGPF FWMFVDNLVLNKEDLDVASRFLEME PVTIPDVHGGSLQNAVRVWSNIPAI RSRHWALVSEEELSLLAQNK QSSKLAAKWPTKLVKNCFLPLREYF KYFSTELTSSL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways

Gene Information

• Gene Name: DNMT3L DNA methyltransferase 3 like [ Homo sapiens (human) ]
• Official Symbol: DNMT3L
• Synonyms: MGC1090
• Gene ID: 29947
• mRNA Refseq: NM_175867.3
• Protein Refseq: NP_787063.1
• MIM: 606588
• UniProt ID: Q9UJW3